Fresh and cryopreserved ovarian tissue from deceased young donors yields viable follicles

Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue.

OBJECTIVE To develop an efficient technique to preserve primordial follicles from cryopreserved ovarian tissue. DESIGN Frozen-thawed and fresh preantral follicles were mechanically isolated for viability testing, and their morphology was histologically analyzed. SETTING Laboratory of Animal Reproduction, Faculty of Veterinary Medicine and Zootechny, University State of São Paulo, Brazil. ...

Histological evaluation and In situ localization of apoptosis in fresh and cryopreserved ovarian tissue

Objective: To study the feasibility of using combined morphology and terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling (TUNEL) for apoptosis detection and the impact of cryopreservation on this process. Materials and methods: We conducted this investigation using a porcine animal model. Bilateral oophorectomy was performed in eight sows. The ovarian tissues were ...

Sex Steroid Production from Cryopreserved and Reimplanted Ovarian Tissue

In Vitro Activation of Follicles and Fresh Tissue Auto-transplantation in Primary Ovarian Insufficiency Patients

CONTEXT Recently, two patients with primary ovarian insufficiency (POI) delivered healthy babies after in vitro activation (IVA) treatment followed by auto-transplantation of frozen-thawed ovarian tissues. OBJECTIVE This study sought to report the first case of live birth after IVA treatment following fresh ovarian tissue grafting in patients with POI, together with monitoring of follicle dev...

In vitro development of secondary follicles from cryopreserved rhesus macaque ovarian tissue after slow-rate freeze or vitrification.

BACKGROUND Ovarian tissue cryopreservation is the only option for preserving fertility in prepubertal girls and cancer patients requiring immediate treatment. Following ovarian tissue cryopreservation, fertility can be restored after tissue transplant or in vitro follicle maturation. METHODS Macaque (n= 4) ovarian cortex was cryopreserved using slow-rate freezing (slow freezing) or vitrificat...